CHAP. III. A DESCRIPTION OF THE MOST APPROVED MICROSCOPES, AND THE METHOD OF USING THEM. (continued 2)

When you view an object, push the ivory slider, in which the said object is placed, between the two flat brass plates, observing always to put that side of the slider, where the brass rings are, farthest from the eye; then screw in the magnifying glass you intend to use at the end of the instrument G, and looking through it against the light, turn the long screw, C C, till your object is brought to appear distinct, or to the true focal distance. To examine any object accurately, view it first through a magnifier that will shew the whole at once, and afterwards inspect the several parts more particularly with one of the greatest magnifiers; for thus you will gain a true idea of the whole, and all its parts: and, though the greatest magnifiers can shew but a minute portion of any object at once, such as the claw of a flea, the horn of a louse, &c. yet by gently moving the slider that contains your object, the eye will gradually see the whole; and if any part should be out of the focal distance, the screw, C C, will easily bring it to the true focus. As objects must be brought very near the glass, when the greatest magnifiers are used, be particularly careful not to rub the slider against the glasses as you move it in or out. A few turns of the screw, C C, will easily obviate this.

DESCRIPTION OF A SCROLL FOR FIXING WILSON’S POCKET MICROSCOPE, AND A MIRROR FOR REFLECTING LIGHT INTO IT.

A B C, Fig. 2, is a brass scroll, which, for the better conveniency of carriage, is made to unscrew into three parts, and may be put into the drawer upon which it stands, with its reflecting mirror D, and Wilson’s pocket microscope, G. The upper part of the scroll is taken off at B, by unscrewing half a turn of the screw; then, if lifted up, it will come out of the socket. The lower part unscrews at C, and the base at E. The mirror lifts out at F, which, with the scroll, lies in one partition of the box.

To apply this scroll for use, fix the body of the microscope to the top thereof by the screw, A, as in Fig. 2, by screwing it in the same hole as the ivory handle was applied to before. The brass or ivory slider being fixed as before described, and the microscope placed in a perpendicular position, move the mirror, D, in such a manner as to reflect the light of the sky, of the sun, or a candle, directly upwards through the microscope; by which means the object will be most conveniently viewed. It is further useful for viewing opake objects, by screwing the arm, Q R, Fig. 1, into the body of the microscope at G; then screwing into the round hole, R, that magnifier which you think will best suit your object, and putting the concave speculum, S, on the outside of the ring, R, you will observe in the body of the microscope, between the wood or brass, F, and the end of the male screw, C C, a small hole, u, through which slides the long wire, T, which has a point at one end, and forceps at the other, that may be used occasionally as your objects require. When you have fixed this, and your object on it, turn the arm, R, till the magnifier is brought over the object; it may be then adjusted to the true focus, by turning the screw, as before. It must also be brought exactly over the speculum, by turning the upper part of the scroll to one side, till your object and the two specula are in one line, as will be found by trial; and then fix it by the screw, B, at which time the upper surface of the object will be enlightened by the light reflected from the mirror, D, to the concave speculum.

DESCRIPTION OF A SMALL MICROSCOPE FOR OPAKE OBJECTS. Plate II. B. Fig. 3 and 4.

A, Fig. 3, is a fixed arm, through which passes a screw, B, the other end is fastened to the moveable arm, C. D, a nut fitted to the said screw, which, when turned, will either separate or bring together the two arms, A C. E, a steel spring, that separates the two sides when the nut is unscrewed. F, a piece of brass turning round in a spring socket, moving on a rivet, in which moves a steel wire pointed at the end G, and the other end a pair of pliers, H: these are either to thrust into, or take up and hold any object, and may be turned round as required. I, a ring of brass, with a female screw fixed on an upright piece of the same metal, turning on a rivet, that it may be set at a due distance when the least magnifiers are used, and is adapted to the screws of all the magnifiers.

Fig. 4, K, a concave speculum of polished silver, in the center of which a lens is placed. On the back of this speculum a male screw, L, is made to fit the brass ring I, Fig. 3. Four of these specula of different concavities, with four glasses of different magnifying powers, as the objects may require. The greatest magnifiers have the least apertures. M, a round object plate, one side white and the other black, intended to render objects the more visible, by placing them, if black, upon the white, and if white, on the black side. A steel spring, N, turns down on each side, to secure any object; from the object plate there is a hollow pipe, to screw it on the needle’s point G, Fig. 3. O, a small box of brass, with a glass on each side, to confine any living object in order to examine it, having a pipe to screw upon the end of the needle at G. P, an ivory handle. Q, a pair of pliers to take up any object. R, a soft hair brush.

To view any object, screw the speculum, with the magnifier you intend to use, into the brass ring, I; place your object either on the needle G, in the pliers H, on the object plate M, or in the brass hollow box O, as may be most convenient; then holding up your instrument by the handle P, look against the light through the magnifying lens, and by means of the nut, D, together with moving of the needle at its lower end, the object may be turned about, raised or depressed, brought nearer the glass, or put farther from it, till you have the true focal distance, and the light be seen reflected from the speculum strongly upon the object.[35]

OF ELLIS’S SINGLE OR AQUATIC MICROSCOPE. Plate VII. B.

This instrument takes its name from Mr. John Ellis, author of “An Essay towards a Natural History of Corallines,” and of the “Natural History of many curious and uncommon Zoophytes.” By this instrument he was enabled to explain many singularities in the œconomy and construction of these wonderful productions of nature. To the practical botanist this instrument is recommended by the respectable authority of Mr. Curtis, author of the Flora Londinensis, a work which does credit to the author and the nation. This microscope is simple in its construction, easy in its use, and very portable; these advantages, as well as some others which it also has over other portable microscopes, have accelerated the sale thereof, and caused it to be very much adopted.

DESCRIPTION OF THE VARIOUS PARTS OF THE MICROSCOPE.

K, the box which contains the whole apparatus; it is generally made of fish-skin; on the top of the box there is a female screw, for receiving the screw which is at the bottom of the brass pillar A, and which is to be screwed on the top of the box, K. D, a brass pin which fits into the pillar; on the top of this pin is a hollow socket to receive the arm which carries the magnifiers; the pin is to be moved up and down, in order to adjust the lenses to their focal or proper distance from the object.

In the representation of this microscope, Plate VII. B. Fig. 1, the pin, D, is delineated as passing through a socket at one side of the pillar, A; it is now usual to make it pass down a hole bored through the middle of the pillar.

E, the bar which carries the magnifying lens; it fits into the socket, X, which is at the top of the pillar, D. This arm may be moved backwards and forwards in the socket X, and sidewise by the pin, D; so that the magnifier, which is screwed into the ring at the end, E, of this bar, may be easily made to traverse over any part of the object lying on the stage or plate B. F is a polished silver speculum, with a magnifying lens placed at the center thereof, which is perforated for this purpose. The silver speculum screws into the arm E, as at F. G, another speculum of a different concavity from the former, with its lens. H, the brass semicircle which supports the mirror, I; the pin, R, affixed to the semicircle, H, passes through the hole which is towards the bottom of the pillar, A. B, the stage or the plane on which the objects are to be placed; it fits into a small dove-tailed arm which is at the upper end of the pillar, A. C, a plane glass, with a small piece of black silk stuck on it; this glass is fitted to a groove made in the stage, B. M, a deep concave glass, to be laid occasionally on the stage instead of the plane glass, C. L, a pair of nippers; these are fixed to the hole of the stage, a, by the pin K; the steel wire of these nippers slides backwards and forwards in the socket, and this socket is moveable upwards and downwards by means of the joint, so that the position of the object may be varied at pleasure. The object may be fixed in the nippers, stuck on the point, or affixed by a little gum-water, &c. to the ivory cylinder, N. O, a small pair of brass forceps to take up minute objects by. P, a brush to clean the glasses.

To use this microscope; begin by screwing the pillar, A, to the cover thereof; pass the pin, R, of the semicircle which carries the mirror through the hole that is near the bottom of the pillar, A; push the stage into the dove-tail at B; slide the pin into the pillar, then pass the bar, E, through the socket, X, which is at the top of the pin D, and screw one of the magnifying lenses into the ring at F.

Now place the object either on the stage, or in the nippers L, and in such a manner, that it may be as nearly as possible over the center of the stage; bring the speculum, F, over the part you mean to observe; then get as much light on the speculum as you can, by means of the mirror, I; the light received on the speculum is reflected by it on the object. The distance of the lens, F, from the object is regulated by moving the pin, D, up and down, until a distinct view of it is obtained. The rule usually observed is, to place the lens beyond its focal distance from the object, and then gradually slide it down, till the object appears sharp and well defined. The adjustment of the lenses to their foci, and the distribution of the light on the object, are what require the most attention.

These microscopes are sometimes fitted up with a rack and pinion to the pillar A, and pin D, for the more ready adjustment of the glasses to their proper foci.

DESCRIPTION OF LYONET’S ANATOMICAL MICROSCOPE. Plate VI. Fig. 3.

Fig. 3 represents the instrument with which M. Lyonet made his microscopical and wonderful dissection of the chenille de saule or caterpillar of the goat moth,[36] of which a specimen is given in Plate XII. Fig. 1, &c. of this work. This portable instrument needs no further recommendation. By it, other observers may be enabled to dissect insects in general with the same accuracy as M. Lyonet, and thus advance the knowledge of comparative anatomy, by which alone the characteristic, nature, and rank of animals, can be truly ascertained.

A B is the anatomical table, which is supported by the pillar O N; this is screwed on the mahogany foot, D C. The table A B, is prevented from turning round by means of two steady pins; in this table or board there is a hole, G, which is exactly over the center of the mirror, F E, that is to reflect the light on the object; the hole, G, is, designed to receive a flat or a concave glass, on which the objects are to be placed that you design to examine or dissect. R X Z is an arm formed of several balls and sockets, by which means it may be moved in every possible position; it is fixed to the board by means of the screw, H; the last arm, I Z, has a female screw, into which a magnifier may be screwed, as at Z. By means of the screw, H, a small motion may be occasionally given to the arm I Z, for adjusting the lens with accuracy to its focal distance from the object. Another chain of balls is sometimes used, carrying a lens to throw light upon the object; the mirror is also so mounted, as to be taken from its place at K, and fitted on a clamp, by which it may be fixed to any part of the table, A B.

To use the dissecting table.

Let the operator sit with his left side near a light window; the instrument being placed on a firm table, the side, D L, towards his breast, the observations should be made with the left eye: this position is well adapted for observing, drawing, or writing. In dissecting, the two elbows are to be supported by the table on which the instrument rests, the hands resting against the board, A B, in order to give it greater stability, as a small shake, though imperceptible to the naked eye, is very visible in the microscope; the dissecting instruments are to be held one in each hand, between the thumb and two fore-fingers. Farther directions are given on the mode of dissecting small objects in the following chapter.

DR. WITHERING’S BOTANICAL MICROSCOPE. Plate VI. Fig. 1.

This small instrument consists of three brass parallel plates, A, B, C; two wires, D and E, are rivetted into the upper and lower plate; the middle plate or stage is moveable on the aforesaid wires, by two little sockets which are fixed to it. The two upper plates each contain a magnifying lens, but of different powers; one of these confines and keeps in their places the fine point F, the forceps G, and the small knife H.

To use this instrument, unscrew the upper lens, and take out the point, the knife, and the forceps; then screw the lens on again, place the object on the stage, and then move it up or down till you have gained a distinct view of the object, as one lens is made of a shorter focus than the other; and spare lenses of a still deeper focus are sometimes added. The principal merit of this microscope is its simplicity.

THE POCKET BOTANICAL AND UNIVERSAL MICROSCOPE.

This pocket instrument is represented at Plate VI. Fig. 2. It is by most naturalists deemed preferable to Dr. Withering’s, being equally simple, more extensive in its application, and the stage unincumbered; though that of M. Lyonet seems better adapted than either to the purposes of dissection only.

A B, a small arm, carrying three magnifiers, two fixed to the upper part, as at B, the other to the lower part of the arm, at C; these may be used separately or combined together, by which you have seven powers. The arm, A B, is supported by the square pillar I K, the lower end of which fits into the socket, E, of the foot, F G; the stage, D L, is made to slide up and down the square pillar. H, a mirror for reflecting light on the object.

To use this microscope, place the object on the stage, L, reflect the light on it from the mirror H, and regulate it to the focus, by moving the stage nearer to or further from the lenses at B C. The ivory sliders pass under the stage, L; other objects may be fixed in the nippers, M N, and then brought under the magnifiers; or they may be laid on one of the glasses fitted to the stage. The apparatus to this instrument consists of three ivory sliders, a pair of nippers, a pair of forceps, a flat glass, and a concave ditto, all fitted to the stage, L. By taking out the pin, M, the pillar, I K, may be turned half round, and the foot, F G, made to answer as an handle.[37]

BOTANICAL MAGNIFIERS.

Since botany has been cultivated with so much ardor, it has been found necessary to contrive some very portable instrument, by which the botanist might investigate the object of his pursuits as it rises before him. Plate VIII. Fig. 7 and 8, represent two of the most convenient sort.

In the tortoiseshell case, Fig. 7, three lenses are contained, d, e, f, of different foci, which are all made to turn into the case, and may be used combined or separately. The three lenses in themselves afford three different magnifying powers; by combining two and two, we make three more; and the three together make, a seventh magnifying power. When the three lenses are used together, it is best to turn them into the case, and look through the hole, for more distinctness, and the exclusion of superfluous light. In the case, Fig. 8, are also three lenses, g, h, i, of different magnifying powers, that all turn up, and shut into the case; but these are not capable of combination.

DESCRIPTION OF A PORTABLE MICROSCOPE AND TELESCOPE. Plate VIII. Fig. 1, to 6.

The telescope is one of those which are composed of several sliding drawers or tubes, for the convenience of being put into the pocket; the sliding tubes are made of thin brass, the outside tube of mahogany. The sliding tubes are contrived to stop, when drawn out to a proper length, so that by applying one hand to the outside tube, and the other hand to the end of the smallest tube, the telescope may at one pull be drawn out to its full length; then any of the tubes (that next the eye is most generally used) may be pushed in gradually, while you are looking through it, till the object is rendered distinct to the eye. To make the tubes slide properly, they all pass through short springs or tubes; these springs may be unscrewed from the ends of the sliding tubes, by means of the milled edges which project above the tubes, and the tubes taken from each other if required, and the springs set closer if at any time they be too weak.

Fig. 5 represents the exterior tube of the telescope, which is to be unscrewed from the rest, at m l, as it does not make any part of the microscope; the cover, k, which protects the object-glass, serves also as a box to contain two ivory wheels, Fig. 1 and 2, with the objects, and a small mirror, Fig. 6.

Fig. 4 is a view of this cover when taken off: unscrew the top part of it, and the mirror, Fig. 6, may be taken out; unscrew the cover of the lower part, and you will find therein the two circular object-wheels above mentioned.

Fig. 3 represents the three internal tubes of the telescope, which constitute the microscopic part thereof. Draw the tubes out in the manner as shewn in the figure; then at the inside, but at the lower end of the exterior tube, a, you will find a short tube, which serves as a stage to hold the object and support the mirror; pull this tube partly out, and turn it, so that a circular hole which is pierced in it may coincide with a similar hole in the exterior tube. This tube is represented as drawn out at Fig. 3, the mirror, Fig. 6, placed therein at b c, and the transparent object-wheel fixed at a.

Fig. 1 represents the slider with transparent objects.

Fig. 2, that with the opake. They are made of ivory, and turn on a pin at the center; the slit end of this pin fits on the edge of the tube, which is then to be pushed up, so that the lower end of the exterior tube may bear lightly on the upper side of the slider, agreeable to the view which is given at a, Fig. 3. Now push down the second tube till the milled part falls on the milled edge of the extreme tube, being careful of the circular hole in the exterior one. Nothing now remains to be done but to adjust for the focus, which is effected by pushing in the tube R, and moving only the first, n.

The instrument may be used in two ways for transparent objects: first, in a vertical position, when the light is to be thrown on the object by the mirror, b c; or it may be examined by looking up directly at the light; in the latter case the mirror must be taken away. In viewing opake objects the mirror is not used; as much light as possible must be admitted on them through the circular holes of the tubes. Any object may be viewed by first pushing in the tube, R, and then bringing the tube, n, to its focal distance from the object. The telescope, when shut up, is about eight inches in length, and when drawn out, is about twenty inches. It is of the achromatic construction.

DESCRIPTION OF AN INSTRUMENT FOR CUTTING THIN TRANSVERSE SECTIONS OF WOOD, Plate IX. Fig. 1.

It consists of a wooden base, which supports four brass pillars; on the top of the pillars is placed a flat piece of brass, near the middle of which there is a triangular hole. A sharp knife which moves in a diagonal direction, is fixed on the upper side of the afore-mentioned plate, and in such a manner, that the edge always coincides with the surface thereof. The knife is moved backwards and forwards by means of the handle, a. The piece of wood is placed in the triangular trough, which is under the brass plate, and is to be kept steady therein by a milled screw which is fitted to the trough; the wood is to be pressed forward for cutting, by the micrometer screw, b. The pieces of wood should be applied to this instrument immediately on being taken out of the ground, or else they should be soaked for some time in water, to soften them, so that they may not hurt the edge of the knife. When the edge of the knife is brought in contact with the piece of wood, a small quantity of spirit of wine should be poured on the surface of the wood, to prevent its curling up; it will also make it adhere to the knife, from which it may be removed by pressing a piece of blotting paper on it.

Fig. 2, is an appendage to the cutting engine, which may be used instead of the micrometer screw, being by some practitioners preferred to it. It is placed over the triangular hole, and kept flat down upon the surface of the brass plate, while the piece of wood is pressed against a circular piece of brass which is on the under side of it. This circular piece of brass is fixed to a screw, by which its distance from the flat plate on which the knife moves may be regulated.[38]