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The Microscope. Its History, Construction, and Application 15th ed

Note 45 — Chapter IV

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With regard to the use of absolute alcohol, this re-agent requires to be used with caution; all minute details are lost, and it causes irregular shrinking of the finer tissues, while fibrous tissue is brought into undue prominence at the expense of the cellular elements. Consequently in certain biological laboratories the method of hardening in alcohol has been abandoned in favour of other re-agents.

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