CONTENTS
Reading edition
Page
I. Laboratory Regulations 1
II. Glass Apparatus in Common Use 3
The Selection, Preparation, and Care of Glassware, 8—Cleaning of Glass Apparatus, 18—Plugging Test-tubes and Flasks, 24.
III. Methods of Sterilisation 26
Sterilising Agents, 26—Methods of Application, 27—Electric Signal Timing Clock, 38.
IV. The Microscope 49
Essentials, 49—Accessories, 57—Methods of Micrometry, 61.
V. Microscopical Examination of Bacteria and Other Micro-fungi 69
Apparatus and Reagents used in Ordinary Microscopical Examination, 69—Methods of Examination, 74.
VI. Staining Methods 90
Bacteria Stains, 90—Contrast Stains, 93—Tissue Stains, 95—Blood Stains, 97—Methods of Demonstrating Structure of Bacteria, 99—Differential Methods of Staining, 108.
VII. Methods of Demonstrating Bacteria in Tissues 114
Freezing Method, 115—Paraffin Method, 117—Special Staining Methods for Sections, 121.
VIII. Classification of Fungi 126
Morphology of the Hyphomycetes, 126—Morphology of the Blastomycetes, 129.
IX. Schizomycetes 131
Anatomy, 134—Physiology, 136—Biochemistry, 144.
X. Nutrient Media 146
Meat Extract, 148—Standardisation of Media, 154—The Filtration of Media, 156—Storing Media in Bulk, 159—Tubing Nutrient Media, 160.
XI. Ordinary or Stock Culture Media 163
XII. Special Media 182
XIII. Incubators 216
XIV. Methods of Cultivation 221
Aerobic, 222—Anaerobic, 236.
XV. Methods of Isolation 248
XVI. Methods of Identification and Study 259
Scheme of Study, 259—Macroscopical Examination of Cultivations, 261—Microscopical Methods, 272—Biochemical Methods, 276—Physical Methods, 295—Inoculation Methods, 315—Immunisation, 321—Active Immunisation, 322—The Preparation of Hæmolytic Serum, 327—The Titration of Hæmolytic Serum, 328—Storage of Hæmolysin, 331.
XVII. Experimental Inoculation of Animals 332
Selection and Care of Animals, 335 —Methods of Inoculation, 352.
XVIII. The Study of Experimental Infections During Life 370
General Observations, 371—Blood Examinations, 373—Serological Investigations, 378—Agglutinin, 381—Opsonin, 387—Immune Body, 393.
XIX. Post-mortem Examination of Experimental Animals 396
XX. The Study of the Pathogenic Bacteria 408
XXI. Bacteriological Analyses 415
Bacteriological Examination of Water, 416—Examination of Milk, 441—Ice Cream, 457—Examination of Cream and Butter, 457—Examination of Unsound Meats, 460—Examination of Oysters and Other Shellfish, 463—Examination of Sewage and Sewage Effluents, 466—Examination of Air, 468—Examination of Soil, 470—Testing Filters, 478—Testing of Disinfectants, 480.
Appendix 492
Index 505
