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Section Cutting and Staining

INDEX.

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Abbe’s condenser, 11 Absolute alcohol, 21 Acetate of copper, 89 Air bubbles, removal of, 56 Alum carmine, 76 hæmatoxyline, 68, 70 Amyloid degeneration, 149 Aniline blue-black, 94 oil, 102, 108 oil water, 107 Apparatus required, 1 Areolar tissue, 133 Bacteria, stains for, 103 Balsam bottle, 58 Barrett’s logwood solution, 69 Bevan Lewis’s method, 94 Bichromate of potassium, 17 Bismarck brown, 104 Bleaching solution, 27 Blood crystals, 130 Blood, methods of examining, 113 Blood-vessels, injection of, 120 Blue injection mass, 121 Bone marrow, 136 Bone, sections of, 136 Borax carmine, 75 Brain, methods of staining, 94 Buckley’s modification of Golgi’s method, 99 Calcareous degeneration, 149 Canada balsam solution, 61 Carbolic acid, 23 Carmine, 74 injection mass, 120 Cathcart microtome, 39 Cathcart-Frazer microtome, 42 Cedar oil, 8, 63 Celloidin, 30 Cementing cover-glasses, 65 Chloral hæmatoxyline, 92 Chloride of gold, 82, 94 Chromic and nitric decalcifying fluid, 26 Ciliated epithelium, 132 Circulation in frog’s foot, 141 Clarifying sections, 63 Clearing agents, 63 Cloudy swelling, 148 Clove oil, 63 Colloid degeneration, 149 Columnar epithelium, 132 Corrosive sublimate hardening, 23 staining, 100 Cover-glasses, cleansing of, 57 Cover-glass preparations, 111 Decalcifying solutions, 26 Dehydration, 63 Eau de Javelle, 27 Ebner’s solution, 27 Ehrlich-Biondi fluid, 85 Ehrlich’s hæmatoxyline, 70 method of fixing blood-films, 116 method for tubercle bacilli, 110 Ehrlich-Gram method for staining bacteria, 108 Elastic cartilage, 135 tissue, 133 Embedding methods, 29 Endothelium, 131 Eosine, 72 Eosine and hæmatoxyline, 73 Epithelial cement, 131 Ether spray microtome, 39 Fæces, staining for bacilli, 112 Farrant’s solution, 59 Fat, removal from sections, 59 staining of, 134 Fatty degeneration, 148 Fearnley’s injection apparatus, 123 Ferrier’s fuchsine solution, 117 Flemming’s solution, 25 Flotation of sections, 55 Folded sections, treatment of, 58 Formal, 23 Fresh sections, 52 Fuchsine, 104 Gentian violet, 104 Gibbes’ stain for tubercle bacilli, 111 Gold chloride, 82, 94 Golgi’s silver method, 96 sublimate method, 99 Gram’s iodide solution, 105 method for staining bacteria, 107 Green injection mass, 122 Gum, 29 Hæmatin crystals, 131 Hæmatoidin, 131 Hæmatoxyline, Ehrlich’s, 70 Kleinenberg’s, 70 Schuchardt’s, 68 Sihler’s, 92 Weigert’s, 88 Hæmoglobin crystals, 130 Hardening processes, 15 Hyaline cartilage, 135 degeneration, 149 Ice freezing microtome, 46 Immersion lenses, 8 Injection of blood-vessels, 120 pulmonary alveoli, 145 Internal ear, 143 Intestines, 146 Iodine solution, 105 Jung’s ether spray microtome, 45 Kleinenberg’s hæmatoxyline, 70 Lardaceous degeneration, 149 Lithio-picrocarmine, 79 Lithium carmine, 74 Liver, 147 Löffler’s methyl blue, 104 Logwood, 68 Lymphoid tissue, 134 Marchi’s fluid, 24 Marrow, 136 Methyl blue, 101, 104 violet, 83 Methylated spirit, 19 Micro-organisms, stains for, 103 Microscope, 6 Microtome, Becker, 49 Cambridge rocking, 49 Cathcart, 39 Cathcart-Frazer, 42 Jung, 45 Schanze, 47 Swift’s, 49 Williams’, 46 Mould for paraffin embedding, 36 Mounting methods, 55 Mucoid degeneration, 148 Muscle, 138 Muir’s method of hardening films, 116 Müller’s fluid, 17 and formal, 20 and spirit, 20 Neelsen’s stain for tubercle bacilli, 110 Nerve cells, stains for, 94 endings, 139 fibres, stains for, 87 Nissl’s aniline method, 101 Nitrate of silver, 82 Nitric acid as hardening agent, 25 decalcifying agent, 26 decolourising agent, 110 Normal salt solution, 53 Nose piece, 9 Objectives, 7 Oil of bergamot, 63 cedar, 8, 63 cloves, 63 origanum, 63 Osmic acid as hardening agent, 21 staining reagent, 81 Pal’s method, 86 solution, 90 Paraffin, 34 Picrocarmine, 78 Pigment cells, 134 Pigmentary degeneration, 150 Plane iron microtome knife, 42 Rapid hardening, 150 Retina, 143 Safranine, 85 Salivary glands, 146 Schäfer-Pal method, 91 Schanze microtome, 47 Schuchardt’s hæmatoxyline, 68 Sihler’s chloral hæmatoxyline, 92 Silver nitrate, stain for nerve cells, 96 epithelial cement, 131 Skin, 142 Spinal cord, 86, 142 Spleen, 147 Sputum, staining of, 111 Squamous epithelium, 131 Staining methods, 67 seq. Stomach, 146 Striped muscle, 138 Sulphuric acid, 105 Sweat glands, 142 Tendon, 133 Testing a microscope, 13 Thymus gland, 145 Thyroid gland, 145 Toison’s fluid, 118 Tooth, sections of, 137 Transitional epithelium, 132 Tubercle bacillus, stains for, 110 Tumours, hardening of, 150 Unstriped muscle, 139 Urine, examination for bacilli, 112 Uterus, 148 Von Ebner’s decalcifying solution, 27 Waxy degeneration, 149 Weigert’s hæmatoxyline method, 88 method for staining bacteria, 106 Williams’ ice freezing microtome, 46 Woodhead’s injection mass, 120 Xylol, 61 Ziehl’s carbol-fuchsine, 105

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