Note 78 — Chapter IV
Reading edition
For the demonstration of flagellæ, the material is taken from a culture on solid media—a young agar culture is best. Make a dilution by Soyka’s method (p. 103), dry and fix on a cover-glass as described, p. 88, then proceed as follows:—
1. Mordant with a mixture of 10 c.c. 20 per cent. tannin solution, 5 c.c. cold saturated ferrous sulphate, 1 c.c. fuchsin or wool-black solution. This solution is put on the preparation, which is then heated for one minute until steam is given off.
2. Wash under the tap, then in alcohol.
3. Stain with anilin-water-fuchsin solution, prepared by dissolving the dye in anilin-water and adding 1 per cent. of a 1 per cent. solution of caustic soda until the liquid begins to go cloudy.
4. Wash off the stain with distilled water.