Note 25 — Chapter II
Reading edition
The cholesterol was obtained by extracting the dry puer with ether, saponifying with alcoholic potash. Shaking out the unsaponifiables with ether, evaporating to dryness. The residue was taken up with absolute alcohol, decolorized with animal charcoal. On cooling, characteristic crystals of cholesterol separated. Hoppe-Seyler finds from 1 per cent. to 4 per cent. cholesterol in fæces. See also Gaultier, Coprologie Clinique, p. 160.